Optimal Syringe Size & Plunger Aspiration Technique to Maximize Cell Recovery
Gronkjaer et al. researched the question of whether a gentle pull of the syringe plunger or a rapid jerk of the plunger creating a rapid negative pressure inside the syringe made a difference in the recovery of cells. They determined that a rapid jerk like motion of the plunger resulted in twice the number of cells compared to gently pulling the plunger. Intuitively this makes sense given the difference in viscosity between blood and marrow. A gentle pull allows the lower viscosity blood to begin to flow leaving the higher viscous marrow intact in the marrow. A rapid jerk of the plunger creates a larger pressure differential at the lumen openings of the needle inside the marrow space. This rapid pressure change does not give the blood a chance to seep into the needle ahead of the marrow.
Hernigou et al. researched whether a larger syringe that creates a greater pressure differential will result in greater cells per ml. For varying volumes of aspirate, they compared a 10ml syringe to a 50ml syringe. They concluded that the optimal syringe size was 10ml combined with a rapid pull of the plunger optimizing the negative pressure. Their rationale for the results was that the resistance of pulling back the 10ml syringe was so much less than the larger syringe. Therefore the user was able to create a more rapid change in pressure using the 10ml syringe even though the larger 50ml syringe was ultimately able to create a greater overall vacuum. Once the lower viscosity peripheral blood begins to flow, the higher vacuum of the 50ml syringe worked at cross-purposes to capturing higher viscous marrow cells. They also reported, regardless of syringe size, that greater volumes of marrow from any one location resulted in fewer cells per ml due to infiltrating peripheral blood. Therefore, the optimal technique is to use a 10ml syringe with a rapid plunger motion and with a 1ml draw from each location.
The Marrow Cellution aspiration system provides a 10ml syringe. We advocate to fill 1ml or no more than 2ml from each aspiration site by pulling plunger and then immediately release it to maximum vacuum. We call this “Snap Back Technique”. The Marrow Cellution Needle is then repositioned and the process repeated.