Frequently Asked Questions
Is KeyPRP a Single or Double Process System and What is the Difference?
The CERVOS KeyPRP System can produce PRP in either a Single or Double Spin Process.
| A “Single Spin” Process creates a “Buffy Coat” Layer between the RBC and Plasma Layers. | A “Double Spin” Process creates a “Buffy Coat” during the Second Spin. The First Spin of the Double Process keeps all Platelets Suspended in Plasma preventing Aggregation (Clumping). |
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Is CERVOS KeyPRP Leukocyte-Rich (LR) or Leukocyte-Poor (LP)?
The CERVOS KeyPRP is considered a Leukocyte-Rich PRP System.
However, it is critical to understand the various Leukocytes/White Blood Cells (WBCs) involved:
CERVOS KeyPRP is Rich in Agranulocytes and Poor in Granulocytes.
Depending on the physician’s desire CERVOS KeyPRP can be customized to produce a Leukocyte-Poor (LP-PRP) or a Leukocyte-Rich PRP (LR-PRP).
What is the Role of Agranulocytes and Granulocytes?
Lighter Agranulocytes (Monocytes, Lymphocytes) are Phagocytic Pathogens (Engulf and Destroy): They fight infection and provide an important anti-microbial protection.
Heavier Granulocytes (Basophils and Eosinophils) are Chemotactic: They attract other cells to migrate to sites of Inflammation and Infection, potentially making the situation worse.
CERVOS KeyPRP is Agranulocyte Rich and Granulocyte Poor!
What is a “Buffy Coat”?
A Buffy Coat is the visible packed Platelet/White Blood Cell Layer after centrifugation.
Buffy Coat PRP Systems utilize different preparation protocols producing varying compositions of Monocyte, Lymphocyte and Neutrophil ratios.
Leukocytes greatly influence the intrinsic biology of acute and chronic tissue conditions because of their immune and host-defense mechanisms. The presence of specific leukocytes in PRP can cause significant cellular and tissue effects.
Eosinophils and Basophils are typically not measurable in PRP formulations as their cell membrane is too fragile to withstand centrifugal force.
What Blood Volume is Required for the KeyPRP System and How much PRP is Obtained?
Each CERVOS KeyPRP Disposable can process as little as 10ml up to 40ml Anticoagulated Blood.
There are markings on each CERVOS KeyPRP Disposable indicating the possibility to produce an End Volume of 2ml, 5ml, or 10ml.
CERVOS KeyPRP Kits are available in Single, Double or Triple Packs.
What is meant by Platelet Recovery and How Many Times Native Platelet Count can be expected with CERVOS KeyPRP?
Platelet Recovery is the Total Number of Platelets in the Final PRP multiplied by the PRP Volume divided by the Total Number of Platelets multiplied by the Total Blood Volume.
Example:
Factor X = Increase Over Native Level
(Data source: Bioscience Research Assoc. Inc ; Nov. 2019)
How do I make ‘Double Spin Process’ PRP with CERVOS KeyPRP Double Kit?
- Fill CERVOS Process Separator (CPD-1) with the desired amount of anticoagulated blood (e.g. 30 ml)
- Process: 2300g for 2 Min. (no Brake!)
- Decant Cloudy Plasma to CERVOS Process Concentrator (CPD-2)
- Process: 2300g for 5 Min. (no Brake!)
- Draw off Excess Plasma, Extract PRP
Abbreviated overview only
How do I make "Single Spin PRP" with CERVOS KeyPRP Kit?
- Fill the CERVOS Process Disposable with the desired amount of anticoagulated blood (e.g. 30 ml)
- Process: 2300g for 2 Min. (no Brake!)
- Draw off Excess Plasma
- Extract residual PRP
Abbreviated overview only
Is Anticoagulant Important?
Yes, Anticoagulant is important to prevent Platelet Aggregation (Clumping).
Typically, ACD-A or Citrate Dextrose Solution (3-4%) is used in a 10% ratio – e.g. 3ml Anticoagulant for 30ml Blood
Is the CERVOS KeyPRP System a "Closed" or "Open" System?
CERVOS KeyPRP is a 100% Closed System.
- CERVOS KeyPRP implements Sterile One-Way Locking Valves.
- CERVOS KeyPRP allows Needleless Operation.
- CERVOS KeyPRP does not require Venting Ports.