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Frequently Asked

The CERVOS PRP System can produce PRP in either a Single or Double Spin Process.

A “Single Spin” Process creates a “Buffy Coat” Layer between the RBC and Plasma Layers. A “Double Spin” Process creates a “Buffy Coat” during the Second Spin.

The First Spin of the Double Process keeps all Platelets Suspended in Plasma preventing Aggregation (Clumping).

The CERVOS KeyPRP is considered a Leukocyte-Rich PRP System.

However, it is critical to understand the various Leukocytes/White Blood Cells (WBCs) involved:
CERVOS KeyPRP is Rich in Agranulocytes and Poor in Granulocytes.

Depending on the physician’s desire CERVOS KeyPRP can be customized to produce a Leukocyte-Poor (LP-PRP) or a Leukocyte-Rich PRP (LR-PRP).

Lighter Agranulocytes (Monocytes, Lymphocytes) are Phagocytic Pathogens (Engulf and Destroy):  They fight infection and provide an important anti-microbial protection.

Heavier Granulocytes (Basophils and Eosinophils) are Chemotactic:  They attract other cells to migrate to sites of Inflammation and Infection, potentially making the situation worse.

CERVOS KeyPRP is Agranulocyte Rich and Granulocyte Poor!

A Buffy Coat is the visible packed Platelet/White Blood Cell Layer after centrifugation.

Buffy Coat PRP Systems utilize different preparation protocols producing varying compositions of Monocyte, Lymphocyte and Neutrophil ratios.

Leukocytes greatly influence the intrinsic biology of acute and chronic tissue conditions because of their immune and host-defense mechanisms. The presence of specific leukocytes in PRP can cause significant cellular and tissue effects.

Eosinophils and Basophils are typically not measurable in PRP formulations as their cell membrane is too fragile to withstand centrifugal force.

Each CERVOS KeyPRP Disposable can process as little as 10ml up to 40ml Anticoagulated Blood.

There are markings on each CERVOS KeyPRP Disposable indicating the possibility to produce an End Volume of 2ml, 5ml, or 10ml.

CERVOS KeyPRP Kits are available in Single, Double or Triple Packs.

Platelet Recovery is the the Total Number of Platelets in the Final PRP multiplied by the PRP Volume divided by the Total Number of Platelets multiplied by the Total Blood Volume.


Factor X = Increase Over Native Level

(Data source: Bioscience Research Assoc. Inc ; Nov. 2019)

  1. Fill 1st CERVOS Process Disposable (CPD-1) with the desired amount of anticoagulated blood (e.g. 30 ml)
  2. Process:  1900g for 2 Min. (no Brake!)
  3. Decant Cloudy Plasma to 2nd CERVOS Process Disposable (CPD-2)
  4. Process:  1900g for 5 Min. (no Brake!)
  5. Draw off Excess Plasma, Extract PRP

Abbreviated overview only

  1. Fill the CERVOS Process Disposable with the desired amount of anticoagulated blood (e.g. 30 ml)
  2. Process:  1900g for 2 Min. (no Brake!)
  3. Draw off Excess Plasma
  4. Extract residual PRP

Abbreviated overview only

Yes, Anticoagulant is important to prevent Platelet Aggregation (Clumping).

Typically, ACD-A or Citrate Dextrose Solution (3-4%) is used in a 10% ratio – e.g. 3ml Anticoagulant for 30ml Blood

CERVOS KeyPRP is a 100% Closed System.

CERVOS KeyPRP implements Sterile One-Way Locking Valves.

CERVOS KeyPRP allows Needleless Operation.

CERVOS KeyPRP does not require Venting Ports.